Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human Adiponectin
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ELISA Detection - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.05-0.2 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of Adiponectin protein ranging from 10,000 to 156 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA Detection: The biotinylated 112-1 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified 62-9 antibody as the capture antibody and human Adiponectin protein as the standard.
For testing human Adiponectin (Acrp30) in serum, plasma, or supernatant, BioLegend’s ELISA LEGEND MAX? Kits are specially developed and recommended.- RRID
- AB_2571888 (BioLegend Cat. No. 528803) AB_2571889 (BioLegend Cat. No. 528804)
Antigen Details
- Structure
- Protein hormone.
- Distribution
- Adipose tissue.
- Function
- Modulates a number of metabolic processes including glucose regulation and fatty acid oxidation.
- Interaction
- Liver, heart, lung, skeletal muscle, and kidney.
- Ligand/Receptor
- Adiponectin receptor 2
- Biology Area
- Complement
- Antigen References
-
1. Ouchi N, et al. 2000. Circulation 102:1296.
2. Ouchi N, et al. 1999. Circulation 100:2473-6.
3. Nakano Y, et al. 1996. J. Biochem. 120:803.
4. Yokota T, et al. 2000. Blood 96:1723. - Gene ID
- 9370 View all products for this Gene ID
- UniProt
- View information about Adiponectin on UniProt.org
