Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- CD70-transfected L cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Fire? 750 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood.
* APC/Fire? 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.- Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications for the relevant formats include: blocking of plasmacytoid dendritic cell induced B cell proliferation and Ig secretion1.
- Application References
(PubMed link indicates BioLegend citation) -
- Shaw J, et al. 2010. Blood 115:3051. (Block)
- RRID
- AB_2820003 (BioLegend Cat. No. 355121) AB_2820004 (BioLegend Cat. No. 355122)
Antigen Details
- Structure
- Type II transmembrane glycoprotein, member of the tumor necrosis factor superfamily, 50 kD
- Distribution
-
Activated T cells and B cells, activated NK cells, activated plasmacytoid dendritic cells, some B cell lymphomas
- Function
- Role in T cell proliferation, generation of cytolytic and memory T cells, B cell proliferation and differentiation
- Ligand/Receptor
- CD27
- Cell Type
- B cells, Dendritic cells, NK cells, T cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Bowman MR, et al. 1994. J. Immunol. 152:1756.
2. Shaw J, et al. 2010. Blood 115:3051.
3. Keller AM, et al. 2009. Blood 113:5167. - Gene ID
- 970 View all products for this Gene ID
- UniProt
- View information about CD70 on UniProt.org
