Product Details
- Verified Reactivity
- Human, Mouse, Rat, All species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH-conjugated His-tag peptide.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1:250-1:2500 (0.2-2ug/mL) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses- Application Notes
This antibody was raised against a linear 8X His tag. It reacts with proteins displaying repeats of 6 Histidines (His6) or more in either internal or C and N terminal regions. It is suitable for ICFC.
Clone J095G46 has been verified for immunocytochemistry (ICC).- RRID
- AB_2716214 (BioLegend Cat. No. 362609)
Antigen Details
- Structure
- 6 repeat His amino acid
- Function
- Epitope tag
- Biology Area
- Cell Biology, Immunology
- Antigen References
-
- Zhao C, et al. 2010. Anal Biochem. 399:237
- Salichs E, et al. 2009. PLoS Genet. 5:e1000397
- Terpe K. 2003. Appl. Microbiol. Biotechnol. 60:523
- Lindner, P, et al. 1997. Biotechniques. 22:140
- Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
- Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
- Gene ID
- NA
- UniProt
- View information about His Tag on UniProt.org
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