Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- In-house recombinant human CCL20
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA. For ELISA capture applications, a concentration of 2.0?g/mL is recommended. To obtain a linear standard curve, serial dilutions of human CCL20 (MIP-3α) recombinant protein ranging from 7.81pg/mL to 500pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.
- Application Notes
ELISA Capture: The purified L2412F11 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated L2418G3 antibody (Cat. No. 534604) as the detection antibody.
Note: For testing human CCL20 (MIP-3a) in cell culture supernatant, serum, plasma (EDTA), and other biological fluids, BioLegend's LEGEND MAX? Kits (Cat. No. 441407 & 441408) are specially developed and recommended.- RRID
- AB_2715819 (BioLegend Cat. No. 534502)
Antigen Details
- Structure
- Protein of 96 amino acid
- Distribution
-
Expression is induced by lipopolysaccharide (LPS) and inflammatory cytokines such as tumor necrosis factor, IL-1β and interferon-γ, and downregulated by IL-10.
- Function
- Involved in the regulation of DC trafficking and the recruitment and activation of T cells. CCL20 also has potent antimicrobial activity.
- Ligand/Receptor
- Ligand for chemokine receptor CCR6.
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Cytokines/Chemokines
- Gene ID
- 6364 View all products for this Gene ID
- UniProt
- View information about CCL20 on UniProt.org
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