Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- In-house recombinant human CCL20
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ELISA Detection - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration of 1.0 ?g/mL is recommended. To obtain a linear standard curve, serial dilutions of human CCL20 (MIP-3α) recombinant protein ranging from 7.81 pg/mL to 500 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.
- Application Notes
ELISA Detection: The biotinylated L2418G3 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified L2412F11 antibody (Cat. No. 534502) as the capture antibody.
Note: For testing human CCL20 (MIP-3a) in cell culture supernatant, serum, plasma (EDTA), and other biological fluids, BioLegend's LEGEND MAX? Kits (Cat. No. 441407 & 441408) are specially developed and recommended.- RRID
- AB_2715820 (BioLegend Cat. No. 534604)
Antigen Details
- Structure
- Protein of 96 amino acid
- Distribution
-
Expression is induced by lipopolysaccharide (LPS) and inflammatory cytokines such as tumor necrosis factor, IL-1β and interferon-γ, and downregulated by IL-10.
- Function
- Involved in the regulation of DC trafficking and recruitment and activation of T cells. CCL20 also has potent antimicrobial activity
- Interaction
- CCR6
- Ligand/Receptor
- Ligand for chemokine receptor CCR6
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Cytokines/Chemokines
- Gene ID
- 6364 View all products for this Gene ID
- UniProt
- View information about CCL20 on UniProt.org
()
