Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- In-house recombinant mouse NGAL
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA - Quality tested
IP - Verified - Recommended Usage
Each lot of this antibody is quality control tested by ELISA. For ELISA capture applications, a concentration of 1.5 ?g/mL is recommended. To obtain a linear standard curve, serial dilutions of NGAL recombinant protein ranging from 1000 to 15.6 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.
- Application Notes
The purified M047A10 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated M0417A8 antibody as the detecting antibody.
For testing mouse NGAL in cell culture supernatant, cell lysate, serum, plasma, urine, and other biological fluids, BioLegend's specially developed LEGEND MAX? Kits are recommended.- RRID
- AB_2650711 (BioLegend Cat. No. 532202)
Antigen Details
- Structure
- 195 amino acid monomer with a predicted molecular weight of approximately 23 kD.
- Distribution
-
Secreted by epithelial cells, macrophages, neutrophils; increased levels have been observed in plasma, serum, and urine in various conditions, such as metastatic breast and colorectal cancer, acute kidney injury, pancreatitis and preeclampsia.
- Function
- Apoptosis, innate immunity, renal development.
- Interaction
- Disulfide-linked with MMP9.
- Ligand/Receptor
- Apoptosis, innate immunity, renal development.
- Cell Type
- Epithelial cells, Macrophages, Neutrophils
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Signal Transduction
- Antigen References
-
1. Iqbal N, et al. 2013. Expert Opin.Med. Diagn. 7:209.
2. Singer E, et al. 2013. Acta. Physiol. (Oxf) 207:663.
3. Palazzuoli A, et al. 2012. Clin. Chim. Acta. 413:1832.
4. Flo TH, et al. 2004. Nature 432:917.
5. Goetz DH, et al. 2002. Mol. Cell. 10:1033.
6. Leung L, et al. 2012. PLoS One 7:e46677.
7. Kjeldsen L, et al. 1993. J. Biol. Chem. 268:10425. - Gene ID
- 16819 View all products for this Gene ID
- UniProt
- View information about NGAL on UniProt.org
