Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human MMP-2, amino acids (Ala30-Cys660) (Accession# NP_004521.1) with a C-terminal His tag and a linker sequence expressed in 293E cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration of 2 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of MMP-2 recombinant protein ranging from 10 to 0.156 ng/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA Capture: The purified M6303D01 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated M6310C11 antibody (Cat. No. 532001) as the detecting antibody.
Note: For testing human MMP-2 in serum or plasma, BioLegend's LEGEND MAX? Kits (Cat. No. 444607 & 444608) are specially developed and recommended.
- Product Citations
-
- RRID
- AB_2566322 (BioLegend Cat. No. 679902)
Antigen Details
- Structure
- Monomer. Human MMP2 contains 660 amino acids with a predicted molecular weight of 73.9 kD. Upon activation by p-aminophenylmercuric acetate (APMA), it has a predicted molecular weight of 62 kD.
- Distribution
-
MMP2 is expressed by fibroblasts, endothelial cells, alveolar epithelial cells, and macrophages.
- Function
- MMP2 is a metalloproteinase that specifically cleaves type IV collagen, which is the major structural component of basement membranes. MMP2 is thought to contribute to the invasion and the metastasis of various human malignancies.
- Interaction
- Fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans, decorin, osteopontin, and IBP-3.
- Ligand/Receptor
- TIMPs, CLL7, and thrombospondin 1.
- Cell Type
- Endothelial cells, Epithelial cells, Fibroblasts, Macrophages
- Biology Area
- Angiogenesis, Cell Adhesion, Cell Biology, Neuroinflammation, Neuroscience
- Molecular Family
- Enzymes and Regulators
- Antigen References
-
1. Nagase H and Woessner JF. 1999. J. Biol. Chem. 274:21491.
2. Martignetti JA, et al. 2001. Nat. Genet. 28:261.
3. Itoh T, et al. 1997. J. Biol. Chem. 272:22389.
4. Passlick B, et al. 2000. Clin. Cancer Res. 6:3944.
5. Qian Q, et al. 2010. Cancer Invest. 28:661.
6. Hoikkala S, et al. 2006. Cancer Lett. 236:125.
7. Yamamura T, et al. 2002. Cancer 35:249. - Gene ID
- 4313 View all products for this Gene ID
- UniProt
- View information about MMP-2 on UniProt.org
