Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human IGFBP-3, amino acids (Gly28-Glu291) (Accession# X64875) with an N-terminal Met, was expressed in 293E cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
- AB_2566243 (BioLegend Cat. No. 679702)
Antigen Details
- Structure
- 291 amino acids with a predicted molecular weight of approximately 32 kD. The glycosylated form migrates at approximately 45-50 kD by SDS-PAGE.
- Distribution
-
IGFBP-3 is expressed by most of the tissues and is present in plasma.
- Function
- Transports insulin like growth factors (IGFs) and protects them from rapid clearance and/or degradation as well as modulating IGFs bioavailability. IGFBP-3 induces apoptosis in vitro and suppresses tumors in vivo. IGFBP-3 is induced by growth hormones, TGF-β, vitamin D, retinoids, and anti-estrogens.
- Interaction
- Interacts with XLKD1, IGF2, IGF1, ALS, HN, and TMEM219.
- Ligand/Receptor
- IGFBP-3R (TMEM219).
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
- Antigen References
-
1. Cohen P, et al. 1992. J. Clin. Endocrinol. Metab. 75:1046.
2. Lee KW and Cohen P. 2002. J. Endocrinol. 175:33.
3. Yan X, et al. 2004. J. Biol. Chem. 279:53232.
4. Shahjee H, et al. 2008. Growth Horm. IGF Res. 18:188-97.
5. Berrigan D, et al. 2009. Growth Horm. IGF Res. 2:146.
6. Ingermann AR, et al. 2010. J. Biol. Chem. 285:30233.
7. Forbes BE, et al. 2012. Front. Endocrinol. 3:38.
8. Baxter RC, et al. 2014. Nat. Rev. Cancer 14:329. - Gene ID
- 3486 View all products for this Gene ID
- UniProt
- View information about IGFBP-3 on UniProt.org
