Product Details
- Verified Reactivity
- Human
- Reported Reactivity
- Cynomolgus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Ig cocktail
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses- Excitation Laser
- Violet Laser (405 nm)
- Application References
(PubMed link indicates BioLegend citation) -
- Lockridge JL, et al. 2013. Biol. Blood Marrow Transplant 9:1310-22. (ELISA)
- Product Citations
-
- RRID
- AB_2561647 (BioLegend Cat. No. 316523) AB_2561648 (BioLegend Cat. No. 316524)
Antigen Details
- Structure
- Ig family
- Distribution
-
B cells
- Cell Type
- B cells
- Biology Area
- Immunology
- Gene ID
- 3514 View all products for this Gene ID
- UniProt
- View information about Ig light chain kappa on UniProt.org