Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Murine T cell hybridoma 2HB51.8
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.
- Preparation
- The antibody was purified by chromatography and conjugated with TotalSeq?-C oligomer under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
PG - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq?-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 ?g per million cells in 100 ?L volume. Refer to the corresponding TotalSeq? protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq? products. For example, for any technology platform Buyer uses with TotalSeq?, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq? with that platform.- Application Notes
Additional reported applications (for the relevant formats) include: Induction of proliferation of V?5.1+ and V?5.2+ T cells2, 3 and in vivo depletion of V?5+ T cells4.
- Additional Product Notes
TotalSeq? reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.
The barcode flanking sequences are CGGAGATGTGTATAAGAGACAGNNNNNNNNNN (PCR handle), and NNNNNNNNNCCCATATAAGA*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Scientific Poster Library.- Application References
(PubMed link indicates BioLegend citation) -
- Kanagawa O, et al. 1991. J. Immunol. 147:1307. (FC)
- Kanagawa O, et al. 1992. J. Immunol. 149:9. (Activ)
- Woodland DL, et al. 1993. J. Exp. Med. 177:433. (Activ)
- Gelber C, et al. 1992. Cancer Res. 52:6507. (Deplete)
- RRID
- AB_2819882 (BioLegend Cat. No. 139519)
Antigen Details
- Structure
- Member of the immunoglobulin superfamily, Vβ5.1and 5.2 are variants of the TCR β chain which associates with the TCR α chain to form the TCR heterodimer.
- Distribution
-
Expressed on a subset of TCR αβ+ T cells.
- Function
- Recognition of peptides presented by the MHC molecules, responsible for T cell mediated immune responses.
- Ligand/Receptor
- MHC-Peptide complex
- Cell Type
- T cells
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
1. Marrack P, et al. 2008. Annu. Rev. Immunol. 26:171.
2. Sim GK and Augustin AA. 1985. Cell 42:89.
3. Mami-Chouaib F, et al. 2002. Immunol. Rev. 188:114. - Gene ID
- 21577 View all products for this Gene ID
- UniProt
- View information about TCR Vb5.1, 5.2 on UniProt.org
