Product Details
- Verified Reactivity
- Human, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- White matter from bovine corpus callosum (from developer)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
ICC, ICFC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on testis samples, a concentration range of 1.25 - 5.0 µg/mL is suggested. For immunohistochemistry on cerebellum samples, a concentration range of 0.5 - 1.25 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone O4 recognizes specifically O4 antigen by immunocytochemistry in oligodendrocytes derived from cortical stem cells using different fixation/permeabilization methods such as methanol, PFA/Triton X-100, and PFA/methanol, where the last method shows the highest signal intensity.
Following immunohistochemistry in paraffin sections, clone O4 showed specific high staining on cerebellum white matter, and very low to none staining in the molecular layer at the recommended concentration.
By flow cytometry staining on differentiated cortical stem cells, clone O4 stained at high intensity complex cells (oligodendrocytes) compared with low complexity cells (undifferentiated progenitor stem cells).- RRID
- AB_2927990 (BioLegend Cat. No. 609551)
AB_2927990 (BioLegend Cat. No. 609552)
Antigen Details
- Structure
- It is a sulfated galactocerebroside (glycosphingolipid), composed of a single galactose linked to a ceramide
- Distribution
Present in the plasma membrane of early immature to mature oligodendrocytes
- Cell Type
- Neural Stem Cells, Oligodendrocytes
- Biology Area
- Neurodegeneration, Neuroscience, Neuroscience Cell Markers
- Antigen References
- Bansal R, et al. 1989. J Neurosci Res. 24:548-57.
- Bansal R, et al. 1999. J Neurosci. 19:7913-24.
- Ahmad I, et al. 2021. PloS One. 16:e0256155.
- Rasti BF, et al. 2020. Int J Nanomedicine. 15:3903-3920.
- Gene ID
- NA
- UniProt
- View information about Oligodendrocyte Marker O4 on UniProt.org
