| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat,Mouse (predicted: GuineaPig,Horse,Cow,Pig,Dog,Human) |
| 產品應用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cathepsin K: 112-210/329 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 | The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. This gene may be subject to RNA editing. [provided by RefSeq, Jul 2008]. Function: Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Subunit: Belongs to the peptidase C1 family. Subcellular Location: Lysosome. Tissue Specificity: Tissue specificityPredominantly expressed in osteclasts (bones). DISEASE: Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature. Similarity: Belongs to the peptidase C1 family. SWISS: P43235 Gene ID: 1513 Database links: Entrez Gene: 1513 Human Entrez Gene: 13038 Mouse Omim: 601105 Human SwissProt: P43235 Human SwissProt: P55097 Mouse Unigene: 632466 Human Unigene: 272085 Mouse Unigene: 5598 Rat 合成與降解（Synthesis and Degradation） 組織蛋白酶K(cathepsinK)是半胱氨酸蛋白酶家族的成員,它和組織蛋白酶S、L和B有很高的同源性。 在心血管方面：組織蛋白酶K參與心血管的很多病理生理過程，在動脈硬化、血管重塑與再生。也是動脈瘤生長、破裂、主動脈辯狹窄進程中主要的膠原酶之一。 在骨與關節方面：組織蛋白酶K主要位于破骨細胞，是涉及骨質再吸收的主要酶，與骨質疏松與脫鈣密切相關。 |
| 產品圖片 |  Sample: Lane 1: Mouse Raw264.7 cell lysates Lane 2: Mouse 4T1 cell lysates Primary: Anti-Cathepsin K (1611R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kDa Observed band size: 46 kDa Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cathepsin K Polyclonal Antibody, Unconjugated(1611R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(S0023) and DAB(C-0010) staining |
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