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產品編號	bs-0032R
英文名稱	Rabbit　Anti-Bcl-2　antibody
中文名稱	Bcl-2抗體
別　　　　名	Apoptosis regulator Bcl 2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; B cell lymphoma 2; Bcl 2; Bcl-2; Bcl2; BCL2 protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma, B-cell, 2; Oncogene B-cell leukemia 2; BCL2_HUMAN.
	Specific References　　(162)　　　　　\|　　　　　bs-0032R has been referenced in 162 publications. [IF=18.027]　Shikai Liu. et al. On-Demand Generation of Peroxynitrite from an Integrated Two-Dimensional System for Enhanced Tumor Therapy. ACS NANO. 2022;XXXX(XXX):XXX-XXX　　WB　;　　Human.　　 PubMed:35666853 [IF=14.593]　Guo-Bin Ding. et al. Molecularly engineered tumor acidity-responsive plant toxin gelonin for safe and efficient cancer therapy. Bioact Mater. 2022 Feb;:　　IHC　;　　Mouse.　　 PubMed:10.1016/j.bioactmat.2022.02.001 [IF=12.153]　Zhang, Fei. et al. Lnc Tmem235 promotes repair of early steroid-induced osteonecrosis of the femoral head by inhibiting hypoxia-induced apoptosis of BMSCs. EXP MOL MED. 2022 Nov;:1-16　　WB　;　　Rat.　　 PubMed:36380019 [IF=9.986]　Jiyoung Hwang. et al. SOD1 suppresses pro-inflammatory immune responses by protecting against oxidative stress in colitis. Redox Biol. 2020 Oct;37:101760　　WB　;　　Human.　　 PubMed:33096425 [IF=8.724]　Yonggang Lv. et al. Three-dimensional decellularized tumor extracellular matrices with different stiffness as bioengineered tumor scaffolds. Bioact Mater. 2021 Sep;6:2767　　WB　;　　Human.　　 PubMed:33665508 [IF=8.702]　Xu Qian. et al. Down-regulation of EVA1A by miR-103a-3p promotes hepatocellular carcinoma cells proliferation and migration. CELL MOL BIOL LETT. 2022 Dec;27(1):1-23　　WB　;　　Human.　　 PubMed:36273122 [IF=8.063]　Luo J et al. ATF4 destabilizes RET through nonclassical GRP78 inhibition to enhance chemosensitivity to bortezomib in human osteosarcoma. Theranostics. 2019 Aug 14;9(21):6334-6353.　　IHC-P　;　　Human.　　 PubMed:31534554 [IF=7.963]　Jianzhao Liao. et al. Gut microbiota disturbance exaggerates battery wastewater-induced hepatotoxicity through a gut-liver axis. Sci Total Environ. 2022 Feb;809:152188　　WB　;　　Mouse.　　 PubMed:34875328 [IF=7.129]　Furui Han. et al. In vivo and in vitro study on hepatotoxicity of Tris-(2, 3-dibromopropyl) isocyanurate exposure via mitochondrial and death receptor pathway. ECOTOX ENVIRON SAFE. 2022 Nov;246:114186　　WB　;　　Rat, Human.　　 PubMed:36244175 [IF=7.129]　Beiyu Zhang. et al. ZnO-NPs alleviate aflatoxin B1-induced hepatoxicity in ducklings by promoting hepatic metallothionein expression. ECOTOX ENVIRON SAFE. 2023 May;256:114826　　WB　;　　Duck.　　 PubMed:36989561 [IF=7.086]　Quan-Kuo He. et al. Captan exposure disrupts ovarian homeostasis and affects oocytes quality via mitochondrial dysfunction induced apoptosis. Chemosphere. 2022 Jan;286:131625　　WB　;　　Mouse.　　 PubMed:34303901 [IF=6.992]　Qingyue Yang. et al. The link between deacetylation and hepatotoxicity induced by exposure to hexavalent chromium. J Adv Res. 2021 Apr;:　　WB　;　　Rat.　　 PubMed:10.1016/j.jare.2021.04.002 [IF=6.819]　Hongchao Xu. et al. A facile method for anti-cancer drug encapsulation into polymersomes with a core-satellite structure. DRUG DELIV. 2022 Jul 29　　IHC　;　　Mouse.　　 PubMed:35904177 [IF=6.792]　Jiayi Li. et al. Toxicological effects of deltamethrin on quail cerebrum: Weakened antioxidant defense and enhanced apoptosis. Environ Pollut. 2021 Oct;286:117319　　WB　;　　Quail.　　 PubMed:33990053 [IF=6.792]　Jiayi Li. et al. Toxicological effects of deltamethrin on quail cerebrum: Weakened antioxidant defense and enhanced apoptosis. Environ Pollut. 2021 Oct;286:117319　　WB　;　　Quail.　　 PubMed:33990053 [IF=6.551]　Wei J et al. Endosulfan induces cardiotoxicity through apoptosis via unbalance of pro-survival and mitochondrial-mediated apoptotic pathways. Sci Total Environ. 2020 Jul 20;727:138790.　　WB　;　　human.　　 PubMed:32344260 [IF=6.543]　Zhou Wan. et al. The Effects of RBP4 and Vitamin D on the Proliferation and Migration of Vascular Smooth Muscle Cells via the JAK2/STAT3 Signaling Pathway. Oxid Med Cell Longev. 2022;2022:3046777　　WB　;　　Rat.　　 PubMed:35082965 [IF=6.434]　Yu D et al. Gestational exposure to acrylamide inhibits mouse placental development in vivo.(2018) J Hazard Mater.367:160-170.　　WB　;　　Mouse.　　 PubMed:30594716 [IF=6.388]　Weiyi Zhang. et al. Cinnamaldehyde induces apoptosis and enhances anti-colorectal cancer activity via covalent binding to HSPD1. PHYTOTHER RES. 2023 Apr;:　　WB　;　　Human.　　 PubMed:37086182 [IF=6.375]　Zhou,et al.CXCR4 antagonist AMD3100 enhances the response of MDA-MB-231 triple-negative breast cancer cells to ionizing radiation.(2018) Cancer Letters. 418:196-203.　　IHC-P + WB　;　　Mouse.　　 PubMed:29317253 [IF=6.304]　Xuemei Shen. et al. CircRILPL1 promotes muscle proliferation and differentiation via binding miR-145 to activate IGF1R/PI3K/AKT pathway. Cell Death Dis. 2021 Feb;12(2):1-14　　WB　;　　Bovine.　　 PubMed:33542215 [IF=6.304]　Zhiqiang Han. et al. PNO1 regulates autophagy and apoptosis of hepatocellular carcinoma via the MAPK signaling pathway. Cell Death Dis. 2021 May;12(6):1-14　　WB　;　　Human.　　 PubMed:34050137 [IF=6.291]　Peng Zheng. et al. Alleviative effect of melatonin on the decrease of uterine receptivity caused by blood ammonia through ROS/NF-κB pathway in dairy cow. Ecotox Environ Safe. 2022 Feb;231:113166　　WB　;　　Bovine.　　 PubMed:35030520 [IF=6.291]　Jia Cui. et al. Effects of ammonia on hypothalamic-pituitary-ovarian axis in female rabbits. Ecotox Environ Safe. 2021 Dec;227:112922　　IHC　;　　Rabbit.　　 PubMed:34700170 [IF=6.217]　Wang L et al. Zoledronic acid inhibits the growth of cancer stem cell derived from cervical cancer cell by attenuating their stemness phenotype and inducing apoptosis and cell cycle arrest through the Erk1/2 and Akt pathways. J Exp Clin?Cancer?Res.?2019 Feb 21;38(1):93.　　WB　;　　Mouse.　　 PubMed:30791957 [IF=6.064]　Mengni Bao. et al. N-Acetylcysteine, an ROS Inhibitor, Alleviates the Pathophysiology of Hyperthyroidism-Induced Cardiomyopathy via the ROS/Ca2+ Pathway. BIOMOLECULES. 2022 Sep;12(9):1195　　WB　;　　Mouse, Rat.　　 PubMed:10.3390/biom12091195 [IF=5.763]　Xu Kejia. et al. Anti-melanoma effect and action mechanism of a novel chitosan-based composite hydrogel containing hydroxyapatite nanoparticles. REGEN BIOMATER. 2022 Jul;:　　IHC　;　　Mouse.　　 PubMed:35958518 [IF=5.682]　Fu, Fang. et al. Ndufa4?Regulates the Proliferation and?Apoptosis?of Neurons via miR-145a-5p/Homer1/Ccnd2. MOL NEUROBIOL. 2023 Feb;:1-18　　WB　;　　Mouse.　　 PubMed:36763283 [IF=5.586]　Shi-Yi Xu. et al. Long-circulating doxorubicin and Schizandrin A liposome with drug-resistant liver cancer activity: in?vitro and in?vivo evaluation. J LIPOSOME RES. 2023 Mar 28　　IHC　;　　Mouse.　　 PubMed:36974767 [IF=5.201]　Xuemei Shen. et al. CircINSR Regulates Fetal Bovine Muscle and Fat Development. Front Cell Dev Biol. 2020; 8: 615638　　WB　;　　Bovine.　　 PubMed:33490079
研究領域	細胞生物　　信號轉導　　細胞凋亡　　細胞類型標志物　　腫瘤細胞生物標志物　　新陳代謝　　線粒體
抗體來源	Rabbit
克隆類型	Polyclonal
交叉反應	Rat,Mouse,Human (predicted: Chicken)
產品應用	WB=1:500-2000, IHC-P=1:100-500, IF=1:100-500, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	26kDa
檢測分子量	26 kDa
細胞定位	細胞核　細胞漿　細胞膜　線粒體
性　　　　狀	Liquid
濃　　　　度	1mg/ml
免　疫　原	KLH conjugated synthetic peptide derived from human Bcl-2:　101-160/236
亞　　　　型	IgG
純化方法	affinity purified by Protein A
緩　沖　液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事項	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產品介紹	BCL2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants (alpha and beta) produced by alternate splicing, differ in their C-terminal ends.BCL2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF1). It can form homodimers, and heterodimers with BAX, BAD, BAK and BclX(L). Heterodimerization with BAX requires intact BH1 and BH2 domains, and is necessary for anti-apoptotic activity. Also interacts with APAF1, RAF1, TP53BP2, BBC3, BCL2L1 and BNIPL. Function: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Subunit: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity. Interacts with EI24 (By similarity). Also interacts with APAF1, BBC3, BCL2L1, BNIPL, MRPL41 and TP53BP2. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets. Interacts with BAG1 in an ATP-dependent manner. Interacts with RAF1 (the 'Ser-338' and 'Ser-339' phosphorylated form). Interacts (via the BH4 domain) with EGLN3; the interaction prevents the formation of the BAX-BCL2 complex and inhibits the anti-apoptotic activity of BCL2. Interacts with G0S2; this interaction also prevents the formation of the anti-apoptotic BAX-BCL2 complex. Subcellular Location: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. Tissue Specificity: Expressed in a variety of tissues. Post-translational modifications: Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A). Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. Monoubiquitinated by PARK2, leading to increase its stability. DISEASE: Note=A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions. Similarity: Belongs to the Bcl-2 family. SWISS: P10415 Gene ID: 596 Database links: Entrez Gene: 281020 Cow Entrez Gene: 596 Human Entrez Gene: 12043 Mouse Entrez Gene: 24224 Rat Omim: 151430 Human SwissProt: O02718 Cow SwissProt: P10415 Human SwissProt: P10417 Mouse SwissProt: P49950 Rat Unigene: 150749 Human Unigene: 257460 Mouse Unigene: 9996 Rat Bcl-2基因是指B-cell lymphoma gene。人體濾泡B細胞淋巴瘤中過量表達的原癌基因。由于染色體t（14；18）易位，將Bcl-2基因置于免疫球蛋白重鏈的轉錄調控下，使其表達失控。在細胞系中其過量表達能延長細胞存活期而不誘導細胞增殖。它是哺乳動物中細胞調亡的抑制基因。參與細胞凋亡的調控。腫瘤中的Bcl-2基因可提高侵潤性瘤細胞的生存能力。主要用于濾胞型淋巴瘤、毛細管性白血病及細胞凋亡等方面的研究。目前研究認為：Bcl-2也是細胞凋亡的一種抑制因子、參與細胞凋亡調控，可以用于各種惡性腫瘤的細胞凋亡的研究。
產品圖片	Protein: Spleen(Mouse)lysate 30ug; Primary: Anti-Bcl-2(bs-0032R) at 1:300; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size : 26kD Observed band size : 26kD Protein: Brain(Mouse)lysate 30ug; Primary: Anti-Bcl-2(bs-0032R) at 1:200; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size : 26kD Observed band size : 26kD Protein: Recombinant protein lysate 100ng; Primary: Anti-Bcl-2(bs-0032R) at 1:200; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size : 26kD Observed band size : 26kD Sample: Lane 1: Mouse Lymph node tissue lysates Lane 2: Mouse Bone tissue lysates Lane 3: Mouse Placenta tissue lysates Lane 4: Rat Spleen tissue lysates Lane 5: Rat Placenta tissue lysates Lane 6: Human K562 cell lysates Lane 7: Human A549 cell lysates Primary: Anti-Bcl-2 (bs-0032R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kDa Observed band size: 27 kDa Sample: MCF-7 (Human) Cell Lysate at 30 ug Hela (Human) Cell Lysate at 30 ug HL60 (Human) Cell Lysate at 30 ug A431 (Human) Cell Lysate at 30 ug Primary: Anti-Bcl-2 (bs-0032R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 23 kD Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-Bcl-2 (bs-0032R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 26 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (bs-0032R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (bs-0032R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (bs-0032R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (bs-0032R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (bs-0032R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (bs-0032R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat ovary tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (bs-0032R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B cell lymphoma 2; Bcl-2) Polyclonal Antibody, Unconjugated (bs-0032R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295G-cy3) for 90 minutes and DAPI for nuclei staining.