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產(chǎn)品編號	bs-1091R
英文名稱	Rabbit　Anti-NADPH oxidase 4　antibody
中文名稱	NADPH氧化酶4抗體
別　　　　名	KOX 1; KOX; Nox 4; Nox-4; NADPH oxidase 4; RENOX; Kidney oxidase-1; Kidney superoxide-producing NADPH oxidase; Kox-1; NADPH; Nox4; NOX4_HUMAN; Renal NAD(P)H-oxidase; RENOX.
	Specific References　　(21)　　　　　\|　　　　　bs-1091R has been referenced in 21 publications. [IF=7.963]　Meiqiong Wu. et al. Suppression of NADPH oxidase 4 inhibits PM2.5-induced cardiac fibrosis through ROS-P38 MAPK pathway. SCI TOTAL ENVIRON. 2022 Apr;:155558　　WB　;　　Mouse,Rat.　　 PubMed:35504386 [IF=7.793]　　　WB,IF　;　　mouse.　　 PubMed:32143149 [IF=7.793]　Wang JN et al. Smad3 promotes AKI sensitivity in diabetic mice via interaction with p53 and induction of NOX4-dependent ROS production. Redox Biol. 2020 Feb 26;32:101479.　　WB　;　　human.　　 PubMed:32143149 [IF=7.419]　Zheng-Hao Sun. et al. Interruption of TRPC6-NFATC1 signaling inhibits NADPH oxidase 4 and VSMCs phenotypic switch in intracranial aneurysm. BIOMED PHARMACOTHER. 2023 May;161:114480　　IHC　;　　Human.　　 PubMed:37002575 [IF=6.691]　Jiang, Wenjuan. et al. Macrophage-derived, LRG1-enriched extracellular vesicles exacerbate aristolochic acid nephropathy in a TGFβR1-dependent manner. 2021 Oct 22　　WB　;　　Human.　　 PubMed:34677723 [IF=6.15]　Hu, Shuang. et al. Deletion of p38γ attenuates ethanol consumption- and acetaminophen-induced liver injury in mice through promoting Dlg1. Acta Pharmacol Sin. 2021 Nov;:1-16　　WB　;　　Mouse.　　 PubMed:34789918 [IF=5.307]　Xu Zhou. et al. Magnesium isoglycyrrhizinate ameliorates lipopolysaccharide-induced liver injury by upregulating autophagy and inhibiting inflammation via IL-22 expression. BIOORG CHEM. 2022 Nov;128:106034　　IHC　;　　Mouse.　　 PubMed:35908353 [IF=5.243]　Peng Ding. et al. BMPER alleviates ischemic brain injury by protecting neurons and inhibiting neuroinflammation via Smad3-Akt-Nrf2 pathway. 2021 Dec 14　　WB　;　　Mouse.　　 PubMed:34904361 [IF=5.237]　Wang JN et al. RIPK1 inhibitor Cpd-71 attenuates renal dysfunction in cisplatin-treated mice via attenuating necroptosis, inflammation and oxidative stress. Clin Sci (Lond). 2019 Jul 25;133(14):1609-1627.　　WB　;　　Mouse&Human.　　 PubMed:31315969 [IF=5.168]　Guo-Jian Jiang. et al. Carteolol triggers senescence via activation of β-arrestin–ERK–NOX4–ROS pathway in human corneal endothelial cells in vitro. CHEM-BIOL INTERACT. 2023 Apr;:110511　　WB　;　　Human.　　 PubMed:37120125 [IF=4.96]　Xue-Qi Liuet al. Rutaecarpine Derivative Cpd-6c Alleviates Acute Kidney Injury by Targeting PDE4B, a Key Enzyme Mediating inflammation in Cisplatin Nephropathy. Biochem Pharmacol. 2020 Oct;180:114132.　　WB　;　　 PubMed:32622666 [IF=4.196]　Takashina Y et al. Sodium Citrate Increases Expression and Flux of Mg Transport Carriers Mediated by Activation of MEK/ERK/c-Fos Pathway in Renal Tubular Epithelial Cells.(2018) Nutrients. Sep 20 ;10(10)　　WB&IP　;　　Rat.　　 PubMed:30241394 [IF=4.102]　Yan Li. et al. Inhibition of Nicotinamide adenine dinucleotide phosphate oxidase 4 attenuates cell apoptosis and oxidative stress in a rat model of polycystic ovary syndrome through the activation of Nrf-2/HO-1 signaling pathway. MOL CELL ENDOCRINOL. Mol Cell Endocrinol. 2022 Apr;:111645　　WB,IHC　;　　Rat.　　 PubMed:35413388 [IF=4.101]　Jing Zhao. et al. Bruton's tyrosine kinase regulates macrophage?induced inflammation in the diabetic kidney via NLRP3 inflammasome activation. Int J Mol Med. 2021 Sep;48(3):1-12　　WB　;　　Mouse.　　 PubMed:34278465 [IF=4.061]　Wang Dan. et al. The Effect of Aerobic Exercise on the Oxidative Capacity of Skeletal Muscle Mitochondria in Mice with Impaired Glucose Tolerance. J DIABETES RES. 2022;2022:3780156　　WB　;　　Mouse.　　 PubMed:35712028 [IF=3.448]　Ma Q et al. Non‐coenzyme role of vitamin B1 in RANKL‐induced osteoclastogenesis and ovariectomy induced osteoporosis. J Cell Biochem. 2020 Feb 26.　　WB　;　　mouse.　　 PubMed:32100911 [IF=3.271]　Pu Tian-Tian. et al. Evaluation of coenzyme Q10 (CoQ10) deficiency and therapy in mouse models of cardiomyopathy. J CARDIOVASC PHARM. 2023 Jan;:10.1097/FJC.0000000000001401　　WB　;　　Mouse.　　 PubMed:36668724 [IF=3.265]　Wang R et al. Leonurine alleviates LPS-induced myocarditis through suppressing the NF-кB signaling pathway. Toxicology. 2019 Apr 18;422:1-13.　　WB　;　　Mouse&Rat.　　 PubMed:31005592 [IF=2.73]　Cheng Yu. et al. Exposure to maternal diabetes induces endothelial dysfunction and hypertension in adult male rat offspring. Microvasc Res. 2021 Jan;133:104076　　WB　;　　Rat.　　 PubMed:32956647 [IF=2.13]　Khan, Aslam, Karen Byer, and Saeed R. Khan. "Exposure of Madin-Darby Canine Kidney (MDCK) Cells to Oxalate and Calcium Oxalate Crystals Activates Nicotinamide Adenine Dinucleotide Phosphate (NADPH)-Oxidase." Urology 83.2 (2014): 510-e1.　　WB　;　　Dog.　　 PubMed:24360063 [IF=0]　Ulmasov et al. CLIC1 null mice demonstrate a role for CLIC1 in macrophage superoxide production and tissue injury. (2017) Physiol.Rep. 5　　IF(ICC)　;　　Mouse.　　 PubMed:28275112
研究領(lǐng)域	細(xì)胞生物　　免疫學(xué)　　信號轉(zhuǎn)導(dǎo)
抗體來源	Rabbit
克隆類型	Polyclonal
交叉反應(yīng)	Human,Mouse,Rat (predicted: Dog,Cow,Horse)
產(chǎn)品應(yīng)用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100, IF=1:100-500, Flow-Cyt=1μg/Test, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	64kDa
細(xì)胞定位	細(xì)胞漿　細(xì)胞膜
性　　　　狀	Liquid
濃　　　　度	1mg/ml
免　疫　原	KLH conjugated synthetic peptide derived from human Nox-4:　81-180/578
亞　　　　型	IgG
純化方法	affinity purified by Protein A
緩　沖　液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng)	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產(chǎn)品介紹	Nox4 is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. Nox4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of Nox4 produces superoxide anions. In vascular cells, the addition of angiotensin II increases Nox4 expression, which suggests a role for Nox-4 in vascular oxidative stress response. Function: Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. Subunit: Interacts with, relocalizes and stabilizes CYBA/p22phox. Interacts with TLR4. Interacts with protein disulfide isomerase. Subcellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction, focal adhesion. Cell membrane. Note=May localize to plasma membrane and focal adhesions. Tissue Specificity: EXpressed in brain, in all layers of the cerebellum, in pyramidal cells of the Ammon horn and in Purkinje cells (at protein level). Expressed in osteoclasts, leukocytes, kidney, liver and lung. Similarity: Contains 1 FAD-binding FR-type domain. Contains 1 ferric oxidoreductase domain. SWISS: Q9JHI8 Gene ID: 50507 Database links: Entrez Gene: 50507 Human Entrez Gene: 50490 Mouse Entrez Gene: 85431 Rat Omim: 605261 Human SwissProt: Q9NPH5 Human SwissProt: Q9JHI8 Mouse SwissProt: Q924V1 Rat Unigene: 371036 Human Unigene: 31748 Mouse Unigene: 14744 Rat 還原型輔酶煙酰胺腺嘌呤二核苷酸磷酸(NADPH)oxidase 4:還原型輔酶煙胺腺嘌呤二核苷酸（Nicotinamide adenine dinucleotide reduced, NADH）位于線粒體膜內(nèi)，是細(xì)胞能量代謝所必需的輔酶，主要功能是經(jīng)電子傳遞鏈產(chǎn)生ATP。NADH在維持細(xì)胞生長、分化和能量代謝中起重要的作用。
產(chǎn)品圖片	Sample: Cerebellum (Mouse) Lysate at 40 ug Primary: Anti-NADPH oxidase 4 (bs-1091R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti- NADPH oxidase4 (bs-1091R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD Sample: HepG2 Cell Lysate at 40 ug Primary: Anti- NADPH oxidase 4(bs-1091R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD Sample: Hela Cell Lysate at 40 ug Primary: Anti- NADPH oxidase 4 (bs-1091R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD Sample: heart (Mouse) Lysate at 40 ug Primary: Anti-NADPH oxidase 4 (bs-1091R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD Sample: muscle (Mouse) Lysate at 40 ug Primary: Anti-NADPH oxidase 4 (bs-1091R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD Sample: Lane 1: Kidney (Mouse) Lysate at 40 ug Lane 2: Lung (Mouse) Lysate at 40 ug Lane 3: Bronchus (Mouse) Lysate at 40 ug Lane 4: Heart (Mouse) Lysate at 40 ug Lane 5: Kidney (Rat) Lysate at 40 ug Lane 6: Lung (Rat) Lysate at 40 ug Lane 7: Bronchus (Rat) Lysate at 40 ug Primary: Anti-NADPH oxidase 4 (bs-1091R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 62 kD Paraformaldehyde-fixed, paraffin embedded (Human kidney ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NADPH oxidase 4) Polyclonal Antibody, Unconjugated (bs-1091R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NADPH oxidase 4) Polyclonal Antibody, Unconjugated (bs-1091R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NADPH oxidase 4) Polyclonal Antibody, Unconjugated (bs-1091R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NADPH oxidase 4) Polyclonal Antibody, Unconjugated (bs-1091R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NADPH oxidase 4) Polyclonal Antibody, Unconjugated (bs-1091R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Nox4 Polyclonal Antibody, Unconjugated(bs-1091R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (Mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NADPH oxidase 4) Polyclonal Antibody, Unconjugated (bs-1091R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NADPH oxidase 4) Polyclonal Antibody, Unconjugated (bs-1091R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining. Blank control: Raji. Primary Antibody (green line): Rabbit Anti-NADPH oxidase 4 antibody (bs-1091R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control:293T. Primary Antibody (green line): Rabbit Anti-NADPH oxidase 4 antibody (bs-1091R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.