| 產品編號 | bs-0608R |
| 英文名稱 | [KO驗證抗體] Rabbit Anti-ICAM1 antibody |
| 中文名稱 | 細胞間粘附分子-1（CD54）抗體 |
| 別 名 | BB2; CD54 antigen; ICAM 1; ICAM-1; Cell surface glycoprotein P3.58; Human rhinovirus receptor; Intercellular adhesion molecule 1; Major group rhinovirus receptor; MALA2; MyD10; P3.58; Surface antigen of activated B cells; ICAM1_HUMAN; MALA-2; MyD10; intercellular adhesion molecule 1 precursor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; major group rhinovirus receptor. |
 | Specific References (24) | bs-0608R has been referenced in 24 publications. |
| 研究領域 | 腫瘤 細胞生物 免疫學 信號轉導 干細胞 細胞膜受體 細胞粘附分子 細胞表面分子 糖蛋白 細胞類型標志物 t-淋巴細胞 b-淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat) |
| 產品應用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 56kDa |
| 檢測分子量 | 85-110kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD54: 201-300/537 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 | This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008] Function: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. Subunit: Homodimer (Probable). Interacts with human herpesvirus 8 MIR2 protein (Probable). Interacts with MUC1 and promotes cell aggregation in epithelial cells. Interacts with ARHGEF26/SGEF. Binds to coxsackievirus A21 capsid proteins and acts as a receptor for this virus. Subcellular Location: Membrane; Single-pass type I membrane protein. Post-translational modifications: Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. Similarity: Belongs to the immunoglobulin superfamily. ICAM family. ontains 5 Ig-like C2-type (immunoglobulin-like) domains. SWISS: P05362 Gene ID: 3383 Database links: Entrez Gene: 3383 Human Entrez Gene: 15894 Mouse Omim: 147840 Human Unigene: 643447 Human SwissProt: P05362 Human SwissProt: P13597 Mouse Unigene: 435508 Mouse Unigene: 12 Rat CD54（ICAM-1）是分子量為60-110KD的膜型糖蛋白，表達于單核和內皮細胞。許多細胞如B或T淋巴細胞、胸腺細胞、纖維母細胞和上皮細胞都可誘導表達CD54，在調節免疫和炎癥反應中CD54有重要作用。 |
| 產品圖片 |  Sample: Hela(Human) Cell Lysate at 30 ug Hela KO ICAM1 (Human) Cell Lysate at 30 ug Primary: Anti-ICAM1/CD54 (bs-0608R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD  Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-ICAM1 (bs-0608R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56/69 kD  Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-ICAM1 (bs-0608R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56/69 kD  Sample: Lymph node (Mouse) Lysate at 40 ug Primary: Anti-ICAM1 (bs-0608R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD  Sample: Raji(Human) Cell Lysate at 30 ug Primary: Anti-CD54 (bs-0608R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD  Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug Lane 2: MCF-7 (Human) Cell Lysate at 30 ug Primary: Anti-ICAM1/CD54 (bs-0608R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110/58 kD Observed band size: 110/58 kD  Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-ICAM1 (bs-0608R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56/69 kD  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD54/ICAM-1 Polyclonal Antibody, Unconjugated(bs-0608R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control (blue line): A431 cells(blue). Primary Antibody (green line): Rabbit Anti-ICAM1/PE-CY7 Conjugated antibody (bs-0608R-PE-CY7) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-PE-CY7 . Protocol The cells were fixed with 70% ice-cold methanol overnight at 4℃ . The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.  Blank control: mouse thymouses(blue) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA(green).  Blank control: HUVEC cells(blue). Primary Antibody:Rabbit Anti-CD54 antibody(bs-0608R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) .Primary antibody (bs-0608R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed. |
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