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產品編號	bs-0572R
英文名稱	Rabbit　Anti-Cyclin B1　antibody
中文名稱	周期素B1抗體
別　　　　名	CCNB 1; CCNB; CCNB1; CCNB1_HUMAN; G2 mitotic specific cyclin B1; G2/mitotic-specific cyclin-B1.
	Specific References　　(21)　　　　　\|　　　　　bs-0572R has been referenced in 21 publications. [IF=7.65]　Zhang L et al. Silica Nanoparticles exacerbates reproductive toxicity development in high-fat diet-treated Wistar rats. J Hazard Mater.?2019 Oct 1:121361.　　WB　;　　Rat.　　 PubMed:31606252 [IF=5.858]　Luchen Sun. et al. Pretreatment of umbilical cord derived MSCs with IFN-γ and TNF-α enhances the tumor-suppressive effect on acute myeloid leukemia. Biochem Pharmacol. 2022 May;199:115007　　WB　;　　Human.　　 PubMed:35307345 [IF=5.01]　Ghate, N. B., et al. "Sundew plant, a potential source of anti-inflammatory agents, selectively induces G2/M arrest and apoptosis in MCF-7 cells through upregulation of p53 and Bax/Bcl-2 ratio." Cell Death Discovery 2 (2016).　　WB　;　　Human.　　 PubMed:27551490 [IF=5.008]　Guo, Hongrui, et al. "Dietary NiCl2 causes cell cycle arrest in the broiler's kidney." Oncotarget. (2015) 6.34:35964-77.　　IHC-P　;　　Chicken.　　 PubMed:26440151 [IF=4.432]　Luya Pu. et al. Icariin arrests cell cycle progression and induces cell apoptosis through the mitochondrial pathway in human fibroblast-like synoviocytes. Eur J Pharmacol. 2021 Dec;912:174585　　WB　;　　Human.　　 PubMed:34678240 [IF=4.101]　Haixiang Ding. et al. Ailanthone suppresses the activity of human colorectal cancer cells through the STAT3 signaling pathway. Int J Mol Med. 2022 Feb;49(2):1-11　　WB　;　　Human.　　 PubMed:34958109 [IF=3.73]　Ghate, Nikhil Baban, et al. "An Antioxidant Extract of Tropical Lichen, Parmotrema reticulatum, Induces Cell Cycle Arrest and Apoptosis in Breast Carcinoma Cell Line MCF-7." PLOS ONE 8.12 (2013): e82293.　　WB　;　　Human.　　 PubMed:24358166 [IF=3.699]　 Zijian Ye . et al. MiR-92b-3p inhibits proliferation and migration of C2C12 cells. Cell Cycle. 2020;19(21):2906-2917　　WB　;　　Mouse.　　 PubMed:33043788 [IF=3.58]　Wang, Qi, et al. "Methamphetamine induces hepatotoxicity via inhibiting cell division, arresting cell cycle and activating apoptosis: In vivo and in vitro studies." Food and Chemical Toxicology (2017).　　WB　;　　Rat.　　 PubMed:28341135 [IF=3.265]　Zeng Q et al.Long-term arsenite exposure induces testicular toxicity by redox imbalance, G2/M cell arrest and apoptosis in mice.(2018) Toxicology. (18)30396-2.　　WB　;　　Mouse.　　 PubMed:30278210 [IF=3.13]　Zhang, Wen-feng, et al. "Angelica polysaccharides inhibit the growth and promote the apoptosis of U251 glioma cells in vitro and in vivo." Phytomedicine (2017).　　WB　;　　Human.　　 PubMed:28887916 [IF=3.098]　Fan Y et al. S5, a Withanolide Isolated from Physalis Pubescens L., Induces G2/M Cell Cycle Arrest via the EGFR/P38 Pathway in Human Melanoma A375 Cells.(2018) Molecules 23(12)　　WB　;　　Human.　　 PubMed:30513793 [IF=2.737]　A K Shendgeet al. A natural flavonoid, apigenin isolated from Clerodendrum viscosum leaves, induces G2/M phase cell cycle arrest and apoptosis in MCF-7 cells through the regulation of p53 and caspase-cascade pathway. Clin Transl Oncol. 2020 Jul 27.　　WB　;　　Human.　　 PubMed:32715386 [IF=2.311]　Hao Zeng. et al. High?throughput sequencing?based analysis of gene expression of hepatitis B virus infection?associated human hepatocellular carcinoma. Oncol Lett. 2020 Oct;20(4):1-1　　IHC　;　　Human.　　 PubMed:32774491 [IF=2.22]　Wang, Chengke, and Zhenxin Wang. "Studying the relationship between cell cycle and Alzheimer’s disease by gold nanoparticle probes." Analytical Biochemistry (2015).　　ELISA　;　　Human.　　 PubMed:26299647 [IF=2.16]　Zhang, Jin, et al. "Silica nanoparticles induce start inhibition of meiosis and cell cycle arrest via down-regulating meiotic relevant factors." Toxicology Research (2016).　　WB　;　　Mouse.　　 PubMed:30090449 [IF=2.08]　Zhang, Jihong, et al. "Interleukin 18 augments growth ability via NF-κB and p38/ATF2 pathways by targeting cylin B1, cyclin B2, cylin A2, and Bcl-2 in BRL-3A rat liver cells." Gene (2015).　　WB　;　　Rat.　　 PubMed:25752290 [IF=1.88]　Maziero RRD et al. Effects of the addition of oocyte meiosis-inhibiting drugs on the expression of maturation-promoting factor components and organization of cytoplasmic organelles. Reprod Biol. 2019 Dec 27. pii: S1642-431X(19)30314-6.　　ICF　;　　bovine.　　 PubMed:31889629 [IF=1.56]　Qu, Zhongyuan, et al. "Chelidonine induces mitotic slippage and apoptotic-like death in SGC-7901 human gastric carcinoma cells." Molecular medicine reports 13.2 (2016): 1336-1344.　　WB　;　　Human.　　 PubMed:26677104 [IF=1.402]　Anil Khushalrao Shendge. et al. The natural flavones, acacetin and apigenin, induce Cdk-Cyclin mediated G2/M phase arrest and trigger ROS-mediated apoptosis in glioblastoma cells. Mol Biol Rep. 2021 Jan;48(1):539-549　　WB　;　　 PubMed:33394232 [IF=0]　NOVIKA RG et al. THE EFFECT OF PSYCHOLOGICAL STRESS ON MPF INTRAFOLLICULAR. International Journal of Applied Pharmaceutics, 2019 127–130.　　IHC　;　　Mouse.　　 PubMed:doi:10.22159/ijap.2019.v11s5.t0102
研究領域	腫瘤　　細胞生物　　免疫學　　細胞周期蛋白
抗體來源	Rabbit
克隆類型	Polyclonal
交叉反應	Human,Mouse,Rat (predicted: Cow)
產品應用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100, IF=1:100-500, Flow-Cyt=1μg/Test, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	48kDa
細胞定位	細胞核　細胞漿
性　　　　狀	Liquid
濃　　　　度	1mg/ml
免　疫　原	KLH conjugated synthetic peptide derived from human Cyclin B1:　271-433/433
亞　　　　型	IgG
純化方法	affinity purified by Protein A
緩　沖　液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事項	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產品介紹	The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]. Function: Essential for the control of the cell cycle at the G2/M (mitosis) transition. Subunit: Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase. Interacts with CCNF; interaction is required for nuclear localization. Subcellular Location: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome. Post-translational modifications: Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site. Similarity: Belongs to the cyclin family. Cyclin AB subfamily. SWISS: P14635 Gene ID: 891 Database links: Entrez Gene: 891　Human Entrez Gene: 268697　Mouse Entrez Gene: 25203　Rat Omim: 123836　Human SwissProt: P14635　Human SwissProt: P24860　Mouse SwissProt: P30277　Rat Unigene: 23960　Human Unigene: 260114　Mouse Unigene: 380027　Mouse Unigene: 482545　Mouse Unigene: 9232　Rat 主要出現在G2期。Cyclin B是細胞周期調節必不可少的條件。細胞周期素B1是細胞周期調節因子，它的異常表達將導致細胞周期發生紊亂，致使腫瘤形成.
產品圖片	Sample: U937 Cell (Human) Lysate at 30 ug Primary: Anti- Cyclin B1 (bs-0572R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 50 kD Sample: Hela Cell (Human) Lysate at 30 ug Primary: Anti- Cyclin B1 (bs-0572R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 50 kD Sample: U251 Cell (Human) Lysate at 30 ug Primary: Anti- Cyclin B1 (bs-0572R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 50 kD Sample: K562 Cell (Human) Lysate at 30 ug Primary: Anti- Cyclin B1 (bs-0572R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 50 kD Sample: Lane 1: Lymph node (Rat) Lysate at 40 ug Lane 2: Testis (Rat) Lysate at 40 ug Lane 3: Spleen (Rat) Lysate at 40 ug Primary: Anti-Cyclin B1 (bs-0572R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55-60 kD Observed band size: 60 kD Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cyclin B1) Polyclonal Antibody, Unconjugated (bs-0572R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cyclin B1 Polyclonal Antibody, Unconjugated(bs-0572R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (Rat esophageal); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cyclin B1) Polyclonal Antibody, Unconjugated (bs-0572R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Cyclin B1) polyclonal Antibody, Unconjugated (bs-0572R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control (blue line): A549 (blue). Primary Antibody (green line): Rabbit Anti-Cyclin B1 antibody（bs-0572R）. Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC. Dilution: 1μg /test. Protocol The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Cell: Hela Concentration:1:100 Host/Isotype:Rabbit/IgG Flow cytometric analysis of primary antibody (Cat#: bs-0572R) on Hela(green) compared with isotype control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .