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產品編號	bs-0244R
英文名稱	Rabbit　Anti-HSP70　antibody
中文名稱	熱休克蛋白70抗體
別　　　　名	HSP70; HSP-70; HSP 70; Heat shock 70 kDa protein 1; heat shock 70kDa protein 1A; Heat shock 70kDa protein 1B; Heat shock induced protein; heat shock protein 70; HSP70 1; HSP70 2; HSP70.1; HSP72; HSPA1; HSPA1A; HSPA1B; XXbac BCX40G17.3 001; Heat shock 70 kDa protein 1A/1B; HSP71A_HUMAN; Hspa1a; Hsp70-1; HSP72; Hspa1; Hspa1b; Heat shock 70 kDa protein 1A/1B; shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2.
	Specific References　　(17)　　　　　\|　　　　　bs-0244R has been referenced in 17 publications. [IF=10.652]　Yanan Hu. et al. Preparation of photothermal responsive and ROS generative gold nanocages for cancer therapy. Chem Eng J. 2021 Oct;421:129744　　WB　;　　Human.　　 PubMed:10.1016/j.cej.2021.129744 [IF=10.435]　Wan, Guoyun. et al. Gene augmented nuclear-targeting sonodynamic therapy via Nrf2 pathway-based redox balance adjustment boosts peptide-based anti-PD-L1 therapy on colorectal cancer. J Nanobiotechnol. 2021 Dec;19(1):1-26　　IF　;　　Mouse.　　 PubMed:34715867 [IF=5.818]　Hehe Du. et al. Cromileptes altivelis microRNA Transcriptome Analysis upon Nervous Necrosis Virus (NNV) Infection and the Effect of cal-miR-155 on Cells Apoptosis and Virus Replication. VIRUSES-BASEL. 2022 Oct;14(10):2184　　FC　;　　Cromileptes altivelis.　　 PubMed:36298739 [IF=5.738]　Lee Jisu. et al. Alpha-2-macroglobulin as a novel diagnostic biomarker for human bladder cancer in urinary extracellular vesicles. FRONT ONCOL. 2022 Sep;0:4727　　WB　;　　Human.　　 PubMed:36176383 [IF=5.696]　Jiang-Tao Fan. et al. Exosomal lncRNA NEAT1 from cancer-associated fibroblasts facilitates endometrial cancer progression via miR-26a/b-5p-mediated STAT3/YKL-40 signaling pathway. Neoplasia. 2021 Jul;23:692　　WB　;　　Human.　　 PubMed:34153644 [IF=5.108]　Wang Y et al. Excessive Cu2+ deteriorates arsenite-induced apoptosis in chicken brain and resulting in immunosuppression, not in homeostasis. Chemosphere.?2019 Sep 4;239:124758.　　WB　;　　Chicken.　　 PubMed:31514009 [IF=4.411]　Hong-Mei Li. et al. Anti-Cancer Properties of Ginkgolic Acids in Human Nasopharyngeal Carcinoma CNE-2Z Cells via Inhibition of Heat Shock Protein 90. Molecules. 2021 Jan;26(21):6575　　WB　;　　Human.　　 PubMed:34770993 [IF=3.74]　Zheng, Jian, et al. "Lithium posttreatment confers neuroprotection through glycogen synthase kinase-3β inhibition in intracerebral hemorrhage rats." Journal of Neurosurgery (2016): 1-9.　　WB　;　　Rat.　　 PubMed:27739937 [IF=3.738]　Wang, Rui. et al. Protective Effect of Manganese on Apoptosis and Mitochondrial Function of Heat-Stressed Primary Chick Embryonic Myocardial Cells. 2021 Nov 15　　WB　;　　Chicken.　　 PubMed:34779997 [IF=3.118]　Liu?J et al. Impacts of simultaneous exposure to arsenic (III) and copper (II) on inflammatory response, immune homeostasis, and heat shock response in chicken thymus. Int Immunopharmacol. 2018 Nov;64:60-68. 　　WB　;　　Chicken.　　 PubMed:30145471 [IF=3.038]　Oyagbemi AA et al. Quercetin attenuates hypertension induced by sodium fluoride via reduction in oxidative stress and modulation of HSP 70/ERK/PPARγ signaling pathways. (2018) Sep 01　　IHC　;　　Rat.　　 PubMed:30171731 [IF=2.984]　Xia Wang. et al. Distinct expression and localization patterns of HSP70 in developmental reproductive organs of rams. Gene. 2020 Nov;760:145029　　WB,IF　;　　Sheep.　　 PubMed:32758578 [IF=2.323]　Xinglin Liu. et al. Effects of Dietary Zearalenone Exposure on the Growth Performance, Small Intestine Disaccharidase, and Antioxidant Activities of Weaned Gilts. Animals-Basel. 2020 Nov;10(11):2157　　WB,IHC　;　　Pig.　　 PubMed:33228146 [IF=2.245]　Dahan Yang. et al. Tandem Mass Tag-Based Quantitative Proteomic Analysis of Chicken Bursa of Fabricius Infected With Reticuloendotheliosis Virus. Front Vet Sci. 2021; 8: 666512　　WB　;　　Chicken.　　 PubMed:34113672 [IF=1.561]　Li L et al. Avian leukosis virus subgroup J and reticuloendotheliosis virus coinfection induced TRIM62 regulation of the actin cytoskeleton. J Vet Sci. 2020 May;21(3):e49.　　WB　;　　Chicken.　　 PubMed:32476322 [IF=1.561]　Ling Li. et al. Avian leukosis virus subgroup J and reticuloendotheliosis virus coinfection induced TRIM62 regulation of the actin cytoskeleton. J Vet Sci. 2020 May; 21(3): e49　　WB　;　　Chicken.　　 PubMed:32476322 [IF=0]　Zhou M et al. Comparative study of stress response, growth and development of uteri in post‐weaning gilts challenged with zearalenone and estradiol benzoate. J Anim Physiol Anim Nutr (Berl). 2019 Sep 4.　　WB&IHC　;　　Pig.　　 PubMed:31483545
研究領域	腫瘤　　細胞生物　　免疫學　　信號轉導
抗體來源	Rabbit
克隆類型	Polyclonal
交叉反應	Human,Mouse,Rat (predicted: Chicken,Cow,Rabbit,Sheep)
產品應用	WB=1:500-2000, IHC-P=1:100-500, ICC=1:100, Flow-Cyt=1ug/Test, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	70kDa
細胞定位	細胞漿
性　　　　狀	Liquid
濃　　　　度	1mg/ml
免　疫　原	KLH conjugated synthetic peptide derived from human HSP70:　500-600/641
亞　　　　型	IgG
純化方法	affinity purified by Protein A
緩　沖　液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事項	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產品介紹	This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]. Function: In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Subunit: Component of the CatSper complex. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with TSC2. Interacts with IRAK1BP1. Interacts with TERT; the interaction occurs in the absence of the RNA component, TERC, and dissociates once the TERT complex has formed. Interacts with DNAJC7. Interacts with CHCHD3. Subcellular Location: Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Tissue Specificity: HSPA1B is testis-specific. Similarity: Belongs to the heat shock protein 70 family. SWISS: P0DMV8 Gene ID: 3303 Database links: Entrez Gene: 281825　Cow Entrez Gene: 3303　Human Entrez Gene: 3304　Human Entrez Gene: 15511　Mouse Entrez Gene: 193740　Mouse Entrez Gene: 24472　Rat Entrez Gene: 294254　Rat Omim: 140550　Human Omim: 603012　Human SwissProt: Q27975　Cow SwissProt: P0DMV8　Human SwissProt: P0DMV9　Human SwissProt: P17879　Mouse SwissProt: Q61696　Mouse SwissProt: Q07439　Rat Unigene: 274402　Human Unigene: 719966　Human Unigene: 728810　Human Unigene: 1950　Rat Unigene: 228225　Rat HSP-70是細胞受應激原刺激后誘導產生的一組應激蛋白，與腫瘤發生、增殖及分化有關。環境和病生理性應激原均可導致肌體合成一組應急蛋白既熱休克蛋白。許多熱休克蛋白，包括HSP70家族成員，均參與蛋白的變性-復性、折疊-解折疊、運輸-易位、活化-非活化和分泌等過程。HSP70與類固醇受體、肌動蛋白、P53等蛋白密切相關。HSP70還參與熱應激原、細胞毒藥物和其他損傷所引起的應急反應，對機體起一定的保護作用。
產品圖片	Sample: Lane 1: Testis (Mouse) Lysate at 40 ug Lane 2: Epididymis (Mouse) Lysate at 40 ug Lane 3: Seminal vesicle (Mouse) Lysate at 40 ug Lane 4: Liver (Mouse) Lysate at 40 ug Lane 5: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 6: Testis (Rat) Lysate at 40 ug Lane 7: Spleen (Rat) Lysate at 40 ug Lane 8: Hela (Human) Cell Lysate at 30 ug Lane 9: Jurkat (Human) Cell Lysate at 30 ug Lane 10: HepG2 (Human) Cell Lysate at 30 ug Lane 11: A549 (Human) Cell Lysate at 30 ug Lane 12: MCF-7 (Human) Cell Lysate at 30 ug Primary: Anti-HSP70 (bs-0244R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 68 kD Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP70) Polyclonal Antibody, Unconjugated (bs-0244R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP70) Polyclonal Antibody, Unconjugated (bs-0244R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP70) Polyclonal Antibody, Unconjugated (bs-0244R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: U-87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HSP70) polyclonal Antibody, Unconjugated (bs-0244R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control:A549. Primary Antibody (green line): Rabbit Anti-HSP70 antibody (bs-0244R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control:A549. Primary Antibody (green line): Rabbit Anti-HSP70 antibody (bs-0244R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control:293T. Primary Antibody (green line): Rabbit Anti-HSP70 antibody (bs-0244R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.