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產品編號	bsm-33036M
英文名稱	Mouse　Anti-beta-Actin (Loading Control)　antibody
中文名稱	β-肌動蛋白/β-Actin（內參）單克隆抗體
別　　　　名	Beta Actin; beta-Actin; ACTB; Actin cytoplasmic 1; Actin, beta; Beta actin; beta cytoskeletal actin;A X actin like protein; ACTB; Actin cytoplasmic 1; alpha sarcomeric Actin; Actx; Beta cytoskeletal actin; Melanoma X actin; PS1TP5BP1; ACTB_HUMAN.
	Specific References　　(85)　　　　　\|　　　　　bsm-33036M has been referenced in 85 publications. [IF=16.836]　　　WB　;　　rabbit.　　 PubMed:10.1002/adfm.201910691 [IF=11.062]　Xiong Ying. et al. SLC2A12 of SLC2 Gene Family in Bird Provides Functional Compensation for the Loss of SLC2A4 Gene in Other Vertebrates. Mol Biol Evol. 2020 Nov;:　　WB　;　　Sparrow.　　 PubMed:33316072 [IF=10.787]　Xi Zhao. et al. Accumulated cholesterol protects tumours from elevated lipid peroxidation in the microenvironment. REDOX BIOL. 2023 Jun;62:102678　　WB　;　　Mouse.　　 PubMed:36940607 [IF=10.787]　Ya Ya Cao. et al. A catalase inhibitor: Targeting the NADPH-binding site for castration-resistant prostate cancer therapy. REDOX BIOL. 2023 Jul;63:102751　　WB　;　　Human.　　 PubMed:37216701 [IF=9.933]　Yuanyuan Jia. et al. Microfluidic Tandem Mechanical Sorting System for Enhanced Cancer Stem Cell Isolation and Ingredient Screening. 2021 Sep 05　　WB　;　　human.　　 PubMed:34486235 [IF=9.727]　Hongxi Wuet al. Loss of a Negative Feedback Loop between IRF8 and AR Promotes Prostate Cancer Growth and Enzalutamide Resistance. Cancer Res. 2020 Jul 1;80(13):2927-2939.　　WB　;　　Human.　　 PubMed:32341037 [IF=9.473]　Xiao-yan He. et al. Cpd-42 Alleviates Acute Kidney Injury via Targeting RIPK3-mediated Necroptosis. BRIT J PHARMACOL. 2023 May;:　　WB　;　　Human，Mouse.　　 PubMed:37248964 [IF=7.328]　Dongdong Yao. et al. Matrix stiffness regulates bone repair by modulating 12-lipoxygenase-mediated early inflammation. Mat Sci Eng C-Mater. 2021 Sep;128:112359　　WB　;　　Rat.　　 PubMed:34474906 [IF=7.077]　Kai Gao. et al. Analysis of meat color, meat tenderness and fatty acid composition of meat in second filial hybrid offspring of MSTN mutant pigs. MEAT SCI. 2022 Nov;193:108929　　WB　;　　Pig.　　 PubMed:35961126 [IF=7.033]　Chunyan Shao. et al. Chitosan-Coated Selenium Nanoparticles Attenuate PRRSV Replication and ROS/JNK-Mediated Apoptosis in vitro. INT J NANOMED. 2022 Jul;17:3043-3054　　WB　;　　Monkey.　　 PubMed:35832119 [IF=6.832]　Deng, Weiyue. et al. Hair follicle-derived mesenchymal stem cells decrease alopecia areata mouse hair loss and reduce inflammation around the hair follicle. Stem Cell Res Ther. 2021 Dec;12(1):1-11　　WB　;　　Mouse.　　 PubMed:34674748 [IF=6.706]　Yu Wang. et al. Seabuckthorn Reverses High-Fat-Diet-Induced Obesity and Enhances Fat Browning via Activation of AMPK/SIRT1 Pathway. NUTRIENTS. 2022 Jan;14(14):2903　　WB　;　　Mouse.　　 PubMed:35889860 [IF=6.304]　Feng Y et al. Long noncoding RNA HOXA-AS2 functions as an oncogene by binding to EZH2 and suppressing LATS2 in acute myeloid leukemia (AML)Cell Death Dis.2020 Dec 2;11(12):1025.　　WB　;　　Human.　　 PubMed:33268767 [IF=6.208]　Bin Zhang. et al. Combined Omics Analysis Further Unveils the Specific Role of Butyrate in Promoting Growth in Early-Weaning Animals. INT J MOL SCI. 2023 Jan;24(2):1787　　WB　;　　Rabbit.　　 PubMed:36675302 [IF=5.818]　Fan Zhang. et al. Analysis of the Function of LncRNA-MSTRG.16919.1 in BHV-1-infected Bovine Kidney Subculture Cells by Transcriptome Sequencing. VIRUSES-BASEL. 2022 Oct;14(10):2104　　WB　;　　Bovine.　　 PubMed:10.3390/v14102104 [IF=5.572]　Jiayuan Luo. et al. Effects of saponins isolated from Polygonatum sibiricum on H2O2-induced oxidative damage in RIN-m5F cells and its protective effect on pancreas. FOOD CHEM TOXICOL. 2023 May;175:113724　　WB　;　　Rat.　　 PubMed:36935075 [IF=5.5]　Tang et al. Co-Upregulation of 14-3-3ζ and P-Akt is Associated with Oncogenesis and Recurrence of Hepatocellular Carcinoma. (2018) Cell.Physiol.Biochem. 45:1097-1107　　WB　;　　Mouse.　　 PubMed:29439255 [IF=5.35]　Shuangshuang Kang. et al. Transcriptional regulation of telomeric repeat-containing RNA by acridine derivatives. 2021 Mar 22　　WB　;　　Human.　　 PubMed:33749516 [IF=5.246]　Wenxuan Jiao. et al. Therapeutic Effects of an Inhibitor of Thioredoxin Reductase on Liver Fibrosis by Inhibiting the Transforming Growth Factor-β1/Smads Pathway. Front Mol Biosci. 2021; 8: 690170　　WB　;　　Mouse.　　 PubMed:34540892 [IF=5.206]　Feng Y et al. LncRNA NR-104098 Inhibits AML Proliferation and Induces Differentiation Through Repressing EZH2 Transcription by Interacting With E2F1. Front Cell Dev Biol. 2020 Mar 26;8:142.　　WB　;　　Human.　　 PubMed:32296698 [IF=5.201]　Feng Y et al. LncRNA NR-104098 Inhibits AML Proliferation and Induces Differentiation Through Repressing EZH2 Transcription by Interacting With E2F1. Front Cell Dev Biol. 2020 Mar 26;8:142.　　WB　;　　human.　　 PubMed:32296698 [IF=5.174]　Xiong Y et al. Physiological and genetic convergence supports hypoxia resistance in high-altitude songbirdsPLoS Genet.2020 Dec 28;16(12):e1009270.　　WB　;　　Bird.　　 PubMed:33370292 [IF=5.076]　Chao Zhang. et al. Affibody Modified G-quadruplex DNA Micelles Incorporating Polymeric 5-Fluorodeoxyuridine for Targeted Delivery of Curcumin to Enhance Synergetic Therapy of HER2 Positive Gastric Cancer. Nanomaterials-Basel. 2022 Jan;12(4):696　　WB　;　　Human.　　 PubMed:10.3390/nano12040696 [IF=5.064]　Fang Wang. et al. Stratifin promotes renal dysfunction in ischemic and nephrotoxic AKI mouse models via enhancing RIPK3-mediated necroptosis. Acta Pharmacol Sin. 2021 Apr;:1-12　　WB　;　　Human, Mouse.　　 PubMed:33833407 [IF=4.658]　Feng Y et al. E2F4 functions as a tumour suppressor in acute myeloid leukaemia via inhibition of the MAPK signalling pathway by binding to EZH2. J Cell Mol Med. 2020 Jan 14.　　WB　;　　Human.　　 PubMed:31943751 [IF=4.486]　Zhuohong Li. et al. Anlotinib exerts anti-cancer efficiency on lung cancer stem cells in vitro and in vivo through reducing NF-κB activity. 2021 May 06　　WB　;　　Mouse.　　 PubMed:33955683 [IF=4.483]　Bingxiang Wang. et al. Atherosclerosis-associated hepatic secretion of VLDL but not PCSK9 is dependent on cargo receptor protein Surf4. J Lipid Res. 2021 Jun;:100091　　WB　;　　Mouse.　　 PubMed:34118252 [IF=4.451]　Yao Lu. et al. Preventive effects of donkey milk powder on the ovalbumin-induced asthmatic mice. J Funct Foods. 2021 Sep;84:104603　　WB　;　　Mouse.　　 PubMed:10.1016/j.jff.2021.104603 [IF=4.411]　Guodong Zhao. et al. Isolation and Characterization of Natural Nanoparticles in Naoluo Xintong Decoction and Their Brain Protection Research. Molecules. 2022 Jan;27(5):1511　　WB　;　　Rat.　　 PubMed:35268609 [IF=4.225]　Yang Liu. et al. Insulin Resistance and Pellino-1 Mediated Decrease in the Activities of Vasodilator Signaling Contributes to Sunitinib-Induced Hypertension. Front Pharmacol. 2021; 12: 617165　　WB　;　　Rat.　　 PubMed:33841146
產品類型	內參抗體
研究領域	腫瘤　　細胞生物　　信號轉導　　細胞骨架
抗體來源	Mouse
克隆類型	Monoclonal
克　隆　號	1A2
交叉反應	Human,Mouse,Rat (predicted: Chicken,Dog,Pig,Cow,Sheep,Fish,GuineaPig,Hamster,Cat)
產品應用	WB=1:5000-20000, IHC-P=1:200-1000, IHC-F=1:200-1000, ICC=1:100, IF=1:200-500, Flow-Cyt=1ug/Test, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	42kDa
細胞定位	細胞漿
性　　　　狀	Liquid
濃　　　　度	1mg/ml
免　疫　原	Synthetic MAP peptide derived from human beta-Actin
亞　　　　型	IgG
純化方法	affinity purified by Protein G
緩　沖　液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事項	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產品介紹	Loading Control This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]. Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Subunit: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Subcellular Location: Cytoplasm. cytoskeleton. Tissue Specificity: Ubiquitously expressed in all eukaryotic cells. Post-translational modifications: ISGylated. Oxidation of Met-44 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced. DISEASE: Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed. Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent. Similarity: Belongs to the actin family. SWISS: P60709 Gene ID: 60 Database links: Entrez Gene: 396526 Chicken Entrez Gene: 60 Human Entrez Gene: 11461 Mouse Entrez Gene: 100009272 Rabbit Entrez Gene: 81822 Rat Omim: 102630 Human SwissProt: P60706 Chicken SwissProt: P60712 Cow SwissProt: P60708 Horse SwissProt: P60709 Human SwissProt: P60710 Mouse SwissProt: P29751 Rabbit SwissProt: P60711 Rat SwissProt: P60713 Sheep Unigene: 520640 Human Unigene: 708120 Human Unigene: 727576 Human Unigene: 328431 Mouse Unigene: 391967 Mouse Unigene: 94978 Rat
產品圖片	Sample: 293T Cell (Human) Lysate at 40 ug NIH/3T3 Cell (Mouse) Lysate at 40 ug Muscle (Mouse) Lysate at 40 ug Primary: Anti-β-Actin (bsm-33036M) at 1/5000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Sample: Hela(Human) Cell Lysate at 30 ug NIH/3T3(Mouse) Cell Lysate at 30 ug A431(Human) Cell Lysate at 30 ug Primary: Anti-beta-Actin (bsm-33036M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Sample: NIH/3T3 (Mouse) Cell Lysate at 30 ug Hela (Human) Cell Lysate at 30 ug A549 (Human) Cell Lysate at 30 ug Primary: Anti-beta-Actin (bsm-33036M) at 1/1000~1/10000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Sample: Cerebrum(Mouse) Lysate at 40 ug Lung(Rat) Lysate at 40 ug Primary: Anti-beta-Actin (bsm-33036M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-beta-Actin (bsm-33036M) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Sample: Lovo(Human) Cell Lysate at 30 ug Primary: Anti- beta-Actin (bsm-33036M) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 46 kD Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 3: Lung (Rat) Lysate at 40 ug Lane 4: Lung (Mouse) Lysate at 40 ug Lane 5: MCF-7 (Human) Cell Lysate at 30 ug Lane 6: A431 (Human) Cell Lysate at 30 ug Lane 7: Stomach (Rat) Lysate at 40 ug Lane 8: Stomach (Mouse) Lysate at 40 ug Lane 9: Cerebrum (Rat) Lysate at 40 ug Lane 10: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-beta-Actin (bsm-33036M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (beta-Actin (Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33036M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (beta-Actin) Monoclonal Antibody, Unconjugated (ascites of bsm-33036M) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (beta-Actin (Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33036M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Blank control:Hela. Primary Antibody (green line): Mouse Anti-beta-Actin antibody (bsm-33036M) Dilution: 1ug/Test; Secondary Antibody : Goat anti-Mouse IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.