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產品編號	bs-3787R
英文名稱	Rabbit　Anti-SCD　antibody
中文名稱	固醇酰輔酶A脫氫酶1抗體
別　　　　名	SCD_HUMAN; Stearoyl-CoA desaturase; EC:1.14.19.1; hSCD1; Acyl-CoA desaturase; Delta(9)-desaturase (Delta-9 desaturase); Fatty acid desaturase; FADS5; SCD1; SCDOS; MSTP008;
	Specific References　　(9)　　　　　\|　　　　　bs-3787R has been referenced in 9 publications. [IF=9.685]　Wang, Nan. et al. USP7- and PRMT5-dependent G3BP2 stabilization drives de novo lipogenesis and tumorigenesis of HNSC. CELL DEATH DIS. 2023 Mar;14(3):1-13　　WB　;　　Human.　　 PubMed:36878903 [IF=5.396]　Zhiyun Hao. et al. MicroRNA-432 inhibits milk fat synthesis by targeting SCD and LPL in ovine mammary epithelial cells. Food Funct. 2021 Aug;:　　WB　;　　Sheep.　　 PubMed:10.1039/D1FO01260F [IF=4.081]　Liu Jiayi. et al. Lithium Chloride Promotes Endogenous Synthesis of CLA in Bovine Mammary Epithelial Cells. BIOL TRACE ELEM RES. 2023 Apr;:1-14　　WB　;　　Bovine.　　 PubMed:37099221 [IF=3.738]　Zong, Jinxin. et al. Lithium Chloride Promotes Milk Protein and Fat Synthesis in Bovine Mammary Epithelial Cells via HIF-1α and β-Catenin Signaling Pathways. Biol Trace Elem Res. 2022 Jan;:1-16　　WB　;　　Bovine.　　 PubMed:35080710 [IF=3.701]　Xiaoxia Liu. et al. EGCG regulates fatty acid metabolism of high-fat diet-fed mice in association with enrichment of gut Akkermansia muciniphila. J Funct Foods. 2020 Dec;75:104261　　IHC　;　　Mouse.　　 PubMed:10.1016/j.jff.2020.104261 [IF=3.448]　Yang X et al. miR‐760 exerts an antioncogenic effect in esophageal squamous cell carcinoma by negatively driving fat metabolism via targeting c‐Myc. J Cell Biochem. 2019 Nov 10.　　WB　;　　Human.　　 PubMed:31709636 [IF=3.411]　Zhang Xiangyan. et al. Molecular Hydrogen Inhibits Colorectal Cancer Growth via the AKT/SCD1 Signaling Pathway. BIOMED RES INT. 2022;2022:8024452　　WB,IHC　;　　Human,Mouse.　　 PubMed:35528164 [IF=3.231]　Jing Fan. et al. Chitosan Oligosaccharide Inhibits the Synthesis of Milk Fat in Bovine Mammary Epithelial Cells through AMPK-Mediated Downstream Signaling Pathway. ANIMALS. 2022 Jan;12(13):1692　　WB　;　　Bovine.　　 PubMed:35804595 [IF=3.12]　Yan R et al. PM2. 5 exposure induces age-dependent hepatic lipid metabolism disorder in female mice. journal of environmental science.2019.　　WB　;　　Mouse.　　 PubMed:doi:10.1016/j.jes.2019.10.014
研究領域	腫瘤　　心血管　　免疫學　　信號轉導　　合成與降解　　脂蛋白　　新陳代謝
抗體來源	Rabbit
克隆類型	Polyclonal
交叉反應	Rat,Mouse,Human (predicted: Sheep,Rabbit,Cow,Pig,Dog)
產品應用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100, IF=1:100-500, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	41kDa
細胞定位	細胞漿　細胞膜
性　　　　狀	Liquid
濃　　　　度	1mg/ml
免　疫　原	KLH conjugated synthetic peptide derived from human SCD:　265-359/359
亞　　　　型	IgG
純化方法	affinity purified by Protein A
緩　沖　液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事項	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產品介紹	This gene encodes an enzyme involved in fatty acid biosynthesis, primarily the synthesis of oleic acid. The protein belongs to the fatty acid desaturase family and is an integral membrane protein located in the endoplasmic reticulum. Transcripts of approximately 3.9 and 5.2 kb, differing only by alternative polyadenlyation signals, have been detected. A gene encoding a similar enzyme is located on chromosome 4 and a pseudogene of this gene is located on chromosome 17. [provided by RefSeq, Sep 2015] Function: Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. Subcellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein (Probable). Similarity: Belongs to the fatty acid desaturase family. SWISS: O00767 Gene ID: 6319 Database links: Entrez Gene: 6319 Human Entrez Gene: 20249 Mouse Entrez Gene: 83792 Rat Omim: 604031 Human SwissProt: O00767 Human SwissProt: P13516 Mouse SwissProt: P07308 Rat Unigene: 558396 Human Unigene: 597496 Human 固醇酰輔酶A脫氫酶1(SCD1)是催化細胞中飽和脂肪酸(SFA)轉化為單不飽和脂肪酸(MUFA)的限速酶，在調控脂肪酸代謝、維持能量平衡、調節胰島素敏感性及衰老等方面都發揮著重要作用，近來在肥胖、激素調節、對瘦素的作用等研究受到了重視。
產品圖片	Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCD1) Polyclonal Antibody, Unconjugated (bs-3787R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCD1) Polyclonal Antibody, Unconjugated (bs-3787R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCD1) Polyclonal Antibody, Unconjugated (bs-3787R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCD1) Polyclonal Antibody, Unconjugated (bs-3787R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCD1) Polyclonal Antibody, Unconjugated (bs-3787R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCD1) Polyclonal Antibody, Unconjugated (bs-3787R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCD1) Polyclonal Antibody, Unconjugated (bs-3787R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-SCD1 Polyclonal Antibody, Unconjugated(bs-3787R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (SCD1) polyclonal Antibody, Unconjugated (bs-3787R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.