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產品編號	bs-1172R
英文名稱	Rabbit　Anti-N-cadherin　antibody
中文名稱	N-鈣粘附分子抗體
別　　　　名	Cadherin 2; Cadherin 2 N cadherin neuronal; Cadherin 2 type 1; Cadherin 2 type 1 N cadherin neuronal; cadherin 2 type 1 N-cadherin neuronal; Cadherin2; Calcium dependent adhesion protein neuronal; CD325; CD325 antigen; CDH2; CDHN; CDw325; CDw325 antigen; N cadherin 1; NCAD; Neural Cadherin; Neural Cadherin.
	Specific References　　(33)　　　　　\|　　　　　bs-1172R has been referenced in 33 publications. [IF=9.933]　Xingyi Xu. et al. A Honeycomb-Like Bismuth/Manganese Oxide Nanoparticle with Mutual Reinforcement of Internal and External Response for Triple-Negative Breast Cancer Targeted Therapy. 2021 Jul 23　　WB　;　　Human.　　 PubMed:34297897 [IF=9.584]　Zheng, Bingxin. et al. Siglec-15-induced autophagy promotes invasion and metastasis of human osteosarcoma cells by activating the epithelial–mesenchymal transition and Beclin-1/ATG14 pathway. CELL BIOSCI. 2022 Dec;12(1):1-15　　WB　;　　Human.　　 PubMed:35842729 [IF=8.456]　Zhang D et al. Cell Membrane-Coated Porphyrin Metal-Organic Frameworks for Cancer Cell Targeting and O2-Evolving Photodynamic Therapy. ACS Appl Mater Interfaces. 2019 Oct 30;11(43):39594-39602.　　WB　;　　Human.　　 PubMed:31577410 [IF=7.727]　Xue Wang. et al. Engineered liposomes targeting the gut–CNS Axis for comprehensive therapy of spinal cord injury. J Control Release. 2021 Mar;331:390　　IF　;　　Rat.　　 PubMed:33485884 [IF=7.076]　Mengmeng Niu. et al. Noncanonical TGF-β signaling leads to FBXO3-mediated degradation of ΔNp63α promoting breast cancer metastasis and poor clinical prognosis. Plos Biol. 2021 Feb;19(2):e3001113　　WB　;　　Human.　　 PubMed:33626035 [IF=6.73]　Yue, Huan. et al. LINC02154 promotes the proliferation and metastasis of hepatocellular carcinoma by enhancing SPC24 promoter activity and activating the PI3K-AKT signaling pathway. CELL ONCOL. 2022 May;:1-16　　WB　;　　Human,Mouse.　　 PubMed:35543858 [IF=6.691]　Zhao, Boyuan. et al. Suspension state and shear stress enhance breast tumor cells EMT through YAP by microRNA-29b. 2021 Oct 07　　WB　;　　Human.　　 PubMed:34618275 [IF=6.63]　Guo, Zhi-chen. et al. Porphyromonas gingivalis promotes the progression of oral squamous cell carcinoma by activating the neutrophil chemotaxis in the tumour microenvironment. CANCER IMMUNOL IMMUN. 2022 Dec;:1-17　　WB　;　　Mouse.　　 PubMed:36513851 [IF=6.217]　Zhang J, et al. LGR5, a novel functional glioma stem cell marker, promotes EMT by activating the Wnt/β-catenin pathway and predicts poor survival of glioma patients.(2018) J. Exp. Clin. Cancer Res. Sep 12;37(1):225.　　IHC　;　　Human.　　 PubMed:30208924 [IF=6.196]　Cheng He. et al. Crosstalk of renal cell carcinoma cells and tumor-associated macrophages aggravates tumor progression by modulating muscleblind-like protein 2/B-cell lymphoma 2/beclin 1-mediated autophagy. CYTOTHERAPY. 2022 Oct;:　　WB, IHC, IF　;　　Mouse, Human.　　 PubMed:36244911 [IF=6.023]　Ling Xie. et al. Suppression of GOLM1 by EGCG through HGF/HGFR/AKT/GSK-3β/β-catenin/c-Myc signaling pathway inhibits cell migration of MDA-MB-231. Food Chem Toxicol. 2021 Nov;157:112574　　WB　;　　human.　　 PubMed:34536514 [IF=5.923]　Nanami Nakamura. et al. Possible Action of Olaparib for Preventing Invasion of Oral Squamous Cell Carcinoma In Vitro and In Vivo. Int J Mol Sci. 2022 Jan;23(5):2527　　WB　;　　Mouse.　　 PubMed:10.3390/ijms23052527 [IF=5.572]　Liu H et al. Anti-tubulin agent vinorelbine inhibits metastasis of cancer cells by regulating epithelial-mesenchymal transition. Eur J Med Chem. 2020 Aug 15;200:112332.　　WB　;　　Human.　　 PubMed:32473523 [IF=5.572]　Xu Yang. et al. Deoxynivalenol induces testicular ferroptosis by regulating the Nrf2/System Xc?/GPX4 axis. FOOD CHEM TOXICOL. 2023 May;175:113730　　WB　;　　Mouse.　　 PubMed:36925038 [IF=4.784]　Zheng Wu. et al. FOXD3 suppresses epithelial–mesenchymal transition through direct transcriptional promotion of SMAD7 in esophageal squamous cell carcinoma. 2021 Sep 22　　WB　;　　human.　　 PubMed:34551139 [IF=4.432]　Botao Pan. et al. Vorinostat targets UBE2C to reverse epithelial-mesenchymal transition and control cervical cancer growth through the ubiquitination pathway. Eur J Pharmacol. 2021 Oct;908:174399　　WB　;　　Human.　　 PubMed:34331954 [IF=4.36]　Yuhua Li. et al. Bolbostemma paniculatum (Maxim.) Franquet extract suppresses the development of colorectal cancer through downregulation of PI3K/Akt pathway. J Ethnopharmacol. 2022 Apr;287:114937　　WB　;　　Human.　　 PubMed:34958876 [IF=4.357]　Fei et al. The number of polyploid giant cancer cells and epithelial-mesenchymal transition-related proteins are associated with invasion and metastasis in human breast cancer. (2015) J.Exp.Clin.Cancer.Res. 34:158　　WB　;　　Human.　　 PubMed:26702618 [IF=4.147]　Francesca Salamanna. et al. Development and characterization of a novel human 3D model of bone metastasis from breast carcinoma in vitro cultured. Bone. 2021 Feb;143:115773　　IHC　;　　Human.　　 PubMed:33249322 [IF=4.12]　Wang et al. Kukoamine A inhibits human glioblastoma cell growth and migration through apoptosis induction and epithelial-mesenchymal transition attenuation. (2016) Sci.Rep. 6:36543　　WB　;　　Human.　　 PubMed:27824118 [IF=4.101]　Yu Guo. et al. RepSox effectively promotes the induced differentiation of sheep fibroblasts into adipocytes via the inhibition of the TGF?β1/Smad pathway. Int J Mol Med. 2021 Aug;48(2):1-13　　WB　;　　Sheep.　　 PubMed:34132357 [IF=4.101]　Haixiang Ding. et al. Ailanthone suppresses the activity of human colorectal cancer cells through the STAT3 signaling pathway. Int J Mol Med. 2022 Feb;49(2):1-11　　WB　;　　Human.　　 PubMed:34958109 [IF=4.068]　Hao Cui. et al. Mena as a key enhancer factor of EMT to promote metastasis of human tongue squamous cell carcinoma. ORAL DIS. 2023 May;:　　WB　;　　Human.　　 PubMed:37203597 [IF=3.98]　Liu, Wen-bin, et al. "Aberrant methylation accounts for cell adhesion-related gene silencing during 3-methylcholanthrene and diethylnitrosamine induced multistep rat lung carcinogenesis associated with overexpression of DNA methyltransferases 1 and 3a." Toxicology and applied pharmacology 251.1 (2011): 70-78.　　IHC-P　;　　Rat.　　 PubMed:21163286 [IF=3.493]　Fangyu Chai. et al. Identification of SLC2A3 as a prognostic indicator correlated with the NF-κB/EMT axis and immune response in head and neck squamous cell carcinoma. CHANNELS. 2023;17(1):Article: 2208928　　WB　;　　Human.　　 PubMed:37134043 [IF=3.405]　Wu Y et al. MFAP5 promotes basal-like breast cancer progression by activating the EMT program.Cell Biosci. 2019 Mar 7;9:24.　　WB　;　　Human.　　 PubMed:30899449 [IF=3.391]　Jasmine Ercoliet al. KRIT1 as a possible new player in melanoma aggressiveness. Arch Biochem Biophys. 2020 Sep 30;691:108483.　　WB　;　　Human.　　 PubMed:32735866 [IF=3.26]　Park, Honghyun, Doyun Kim, and Kuen Yong Lee. "Interaction‐tailored cell aggregates in alginate hydrogels for enhanced chondrogenic differentiation."Journal of Biomedical Materials Research Part A (2016).　　IHC-P　;　　Mouse.　　 PubMed:27529335 [IF=3.111]　Qi Rao. et al. Effects of dihydroartemisinin combined with cisplatin on proliferation, apoptosis and migration of HepG2 cells. ONCOL LETT. 2022 Aug;24(2):1-9　　WB　;　　Human.　　 PubMed:35782905 [IF=2.766]　Ning et al. Vascular endothelial growth factor receptor-1 activation promotes migration and invasion of breast cancer cells through epithelial-mesenchymal transition. (2013) PLoS.One. 8:e65217　　IHC-P　;　　Human.　　 PubMed:23776453
研究領域	腫瘤　　細胞生物　　免疫學　　激酶和磷酸酶　　細胞粘附分子
抗體來源	Rabbit
克隆類型	Polyclonal
交叉反應	Human,Mouse,Cow (predicted: Rat,Pig,Danio rerio)
產品應用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100, IF=1:100-500, Flow-Cyt=1:100, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	100kDa
細胞定位	細胞膜
性　　　　狀	Liquid
濃　　　　度	1mg/ml
免　疫　原	KLH conjugated synthetic peptide derived from human N-cadherin:　701-800/905
亞　　　　型	IgG
純化方法	affinity purified by Protein A
緩　沖　液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事項	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產品介紹	This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. Function: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. Subcellular Location: Cell membrane. Similarity: Contains 5 cadherin domains. SWISS: P19022 Gene ID: 1000 Database links: Entrez Gene: 1000 Human Entrez Gene: 281062 Cow Entrez Gene: 12558 Mouse Entrez Gene: 83501 Rat Omim: 114020 Human SwissProt: P19022 Human SwissProt: P15116 Mouse SwissProt: Q9Z1Y3 Rat Unigene: 464829 Human Unigene: 606106 Human Unigene: 257437 Mouse Unigene: 23200 Rat 細胞粘附蛋白（Call Adhesion Protein） N-鈣粘附分子屬于依賴鈣離子的細胞間糖蛋白粘附分子的一部分,主要是參與細胞間同源性粘附,調控和介導細胞間相互反應。
產品圖片	Sample: HepG2 Cell Lysate at 40 ug Eye (Mouse) Lysate at 40 ug Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- N-cadherin (bs-1172R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100kD Observed band size: 110kD Sample: Colon carcinoma(Human) lysate at 30ug; Brain(Rat) lysate at 30ug; Primary: Anti-CDH2/N-cadherin (bs-1172R) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 100kD Observed band size : 100kD Sample: Breast ca (Mouse) Lysate at 40 ug Primary: Anti-N-cadherin (bs-1172R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100 kD Observed band size: 105 kD Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-N-cadherin Polyclonal Antibody, Unconjugated(bs-1172R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-N-cadherin Polyclonal Antibody, Unconjugated(bs-1172R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell:SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (N-cadherin) polyclonal Antibody, Unconjugated (bs-1172R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Tissue/cell:SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (N-cadherin) polyclonal Antibody, Unconjugated (bs-1172R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-N-cadherin Polyclonal Antibody, Unconjugated(bs-1172R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei Blank control: Hepg2(blue). Primary Antibody:Rabbit Anti-E cadherin antibody (bs-1172R,Green); Dilution: 3μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody (bs-1172R, 3μg /1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (1 hour) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. Acquisition of 20,000 events was performed.