| 產品編號 | bs-0295D-AF594 |
| 英文名稱 | Donkey Anti-Rabbit IgG H&L / AF594 antibody |
| 中文名稱 | Alexa Fluor 594標記的驢抗兔IgG H&L |
| 別 名 | Donkey Anti-Rabbit IgG H&L (Alexa Fluor? 594); Immunoglobulin G; |
 | Specific References (2) | bs-0295D-AF594 has been referenced in 2 publications. [IF=22.096] Zhu, Fangrui. et al. The neuropeptide CGRP enters the macrophage cytosol to suppress the NLRP3 inflammasome during pulmonary infection. CELL MOL IMMUNOL. 2023 Jan;:1-13 IF ; Mouse. [IF=6.208] Uliana De Simone. et al. Human Astrocyte Spheroids as Suitable In Vitro Screening Model to Evaluate Synthetic Cannabinoid MAM2201-Induced Effects on CNS. INT J MOL SCI. 2023 Jan;24(2):1421 IF,FCM ; Human. |
| 抗體來源 | Donkey |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rabbit |
| 產品應用 | IF=1:100-1000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 狀 | Liquid |
| 濃 度 | 1.0 mg/ml |
| 免 疫 原 | Native rabbit IgG |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存條件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品介紹 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| 產品圖片 |  Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4°C, followed by a conjugated Rabbit IgG H&L / AF594 antibody (bs-0295D-AF594) for 90 minutes, and DAPI for nuclei staining.  Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4°C, followed by a conjugated Rabbit IgG H&L / AF594 antibody (bs-0295D-AF594) for 90 minutes, and DAPI for nuclei staining.  Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4°C, followed by a conjugated Rabbit IgG H&L / AF594 antibody (bs-0295D-AF594) for 90 minutes, and DAPI for nuclei staining.  Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4°C, followed by a conjugated Rabbit IgG H&L / AF594 antibody (bs-0295D-AF594) for 90 minutes, and DAPI for nuclei staining. |
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